產(chǎn)品[
CCL-75.1 WI－38AV13 SV－40 人轉化肺成纖維細胞
]資料
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產(chǎn)品名稱(chēng):
CCL-75.1 WI－38AV13 SV－40 人轉化肺成纖維細胞
產(chǎn)品型號:
CCL-75.1
產(chǎn)品廠(chǎng)商:
美國標準生物品收藏中心（ATCC）
產(chǎn)品文檔:
無(wú)相關(guān)文檔
簡(jiǎn)單介紹
CCL-75.1 WI－38AV13 SV－40 人轉化肺成纖維細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件！
CCL-75.1 WI－38AV13 SV－40 人轉化肺成纖維細胞
的詳細介紹
CCL-75.1 WI－38AV13 SV－40 人轉化肺成纖維細胞
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CCL-75.1?
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$323.00
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WI-38 VA-13 subline 2RA
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AJ Girardi
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Biosafety Level:
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2 [Cells contain polyomavirus DNA sequences ]
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frozen
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See Propagation
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adherent
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Homo sapiens (human)
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epithelial
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Organ: lung
Cell Type: fibroblastSV40 transformed
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Isolation date: March, 1964
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transfection host (Roche FuGENE® Transfection Reagents)
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Human poliovirus 2
Herpes simplex virus
Vesicular stomatitis virus
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Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
THO1: 9.3
TPOX: 8
vWA: 19,20
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modal number = 76; range = 45 to 89.
Karyotype unstable within hyperdiploid stemline range 73-78. Ninety percent of the cells examined have 2 or 3 large chromosomes with subterminal centromeres and 1-6 microchromosomes. Secondary constrictions were observed in 59% of the cells, 21% had breaks.
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G6PD, B
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3 months gestation
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female
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Caucasian
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
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Protocol:
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Remove and discard culture medium.
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Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
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Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
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Add appropriate aliquots of the cell suspension to new culture vessels.
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Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week
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Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
parental cell line:ATCC CCL-75
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23133: Girardi AJ, et al. SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters. Ann. Med. Exp. Biol. Fenn. 44: 242-254, 1966. PubMed: 4290661
26230: Jensen F, et al. Autologous and homologous implantation of human cells transformed in vitro by simian virus 40. J. Natl. Cancer Inst. 32: 917-937, 1964.
32701: Wang S, Vos JM. A hybrid herpesvirus infectious vector based on epstein-barr virus and herpes simplex virus type 1 for gene transfer into human cells in vitro and in vivo. J. Virol. 70: 8422-8430, 1996. PubMed: 8970963
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