產(chǎn)品[
BNL CL.2 小鼠胚胎肝細胞
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產(chǎn)品名稱(chēng):
BNL CL.2 小鼠胚胎肝細胞
產(chǎn)品型號:
TIB-73
產(chǎn)品廠(chǎng)商:
美國標準生物品收藏中心（ATCC）
產(chǎn)品文檔:
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簡(jiǎn)單介紹
BNL CL.2 小鼠胚胎肝細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件！
BNL CL.2 小鼠胚胎肝細胞
的詳細介紹
BNL CL.2 小鼠胚胎肝細胞
ATCC® Number: TIB-73?
Designations: BNL CL.2
Depositors: P Patek, J Collins, M Cohn
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology:
Source: Organ: liver
Strain: BALB/c
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: No
Age: embryo
Comments: The line was selected to grow in medium containing ornithine and phenylalanine in place of arginine and tyrosine.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for 1 minute, remove trypsin and let the culture sit at room temperature until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Related Products: derivative:ATCC TIB-74
References: 22905: Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934
